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Methyltestosterone Feed Stability and Water Biodegradation Studies


Drug Approval Research on 17a-Methyltestosterone


The objectives for the fourth aquaculture drug project, Drug Approval Research on 17-a Methyltestosterone, include:

1.Develop a robust and validated high performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS) method to measure 17α-methyltosterone (MT) in fish feed.

2.Conduct a series of stability studies on MT in fish feed.

3.Gain acceptance from the Center for Veterinary Medicine (CVM) for the series of stability studies.

4.Review and develop a LC-MS method for detecting MT in water.

5.Conduct a biodegradation study of MT in water.

6.Gain acceptance from CVM for the biodegradation study on MT.


Principal Investigator/Institution Objectives Funding
Ashok K. Marwah and Padma Marwah (University of Wisconsin - Madison) 1 -6 $169,362
Terence P. Barry (University of Wisconsin-Madison) 2, 3, 5 & 6 $ 54,315
                                   Totals   $223,667

Project Outline (115K pdf file)

Termination Report (127K pdf file)

Project Number: 4

Award Amount


Award Number