Description:

We propose to use metagenomic DNA sequencing techniques to characterize populations of microbes in aquaponics systems in the Midwest.

Chaired by Matthew Rogge

Objectives:

1. Collect microbial samples from multiple sites within an aquaponics system and from various systems across a
    geographic range and extract bacterial DNA using commercially-available kits.
2. Submit DNA samples to the UW-Madison Biotechnology Center for PCR amplification, Illumina
    next-generation sequencing, and bioinformatics analyses to compare microbial populations
    among sampling sites within a system, among systems at a single production site, and among
    production sites in the North-Central Region (NCR).
3. Develop a fact sheet explaining the importance of microbial populations in an aquaponics system,
    including the risk that certain organisms can cause (e.g., plant, fish, and human pathogens),
    develop a recorded presentation for dissemination using the NCRAC website, and produce oral
    presentations and a peer-reviewed manuscript.

Participant(s):

Project Outline

Termination Report

Project Number: 1

Award Amount: $24,596

Principal Investigator: Matthew Rogge

Award Amount

$24596.00

Award Number

2014-38500-22138