Metagenomic Analysis of Microbial Populations in Aquaponics Systems



We propose to use metagenomic DNA sequencing techniques to characterize populations of microbes in aquaponics systems in the Midwest.

Chaired by Matthew Rogge


1. Collect microbial samples from multiple sites within an aquaponics system and from various systems across a
    geographic range and extract bacterial DNA using commercially-available kits.
2. Submit DNA samples to the UW-Madison Biotechnology Center for PCR amplification, Illumina
    next-generation sequencing, and bioinformatics analyses to compare microbial populations
    among sampling sites within a system, among systems at a single production site, and among
    production sites in the North-Central Region (NCR).
3. Develop a fact sheet explaining the importance of microbial populations in an aquaponics system,
    including the risk that certain organisms can cause (e.g., plant, fish, and human pathogens),
    develop a recorded presentation for dissemination using the NCRAC website, and produce oral
    presentations and a peer-reviewed manuscript.


Principal Investigators/Institution Objectives Total
Matthew Rogge (Univ. of WI-Stevens Point 1, 2, 3 $18,596
Nicholas Phelps 1, 3 $4,500
Danial Graf 2, 3 $1,500
Total   $24,596

Project Outline

Termination Report

Project Number: 1

Award Amount: $24,596

Principal Investigator: Matthew Rogge

07/01/2016 to 06/30/2017
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