We propose to use metagenomic DNA sequencing techniques to characterize populations of microbes in aquaponics systems in the Midwest.
Chaired by Matthew Rogge
1. Collect microbial samples from multiple sites within an aquaponics system and from various systems across a
geographic range and extract bacterial DNA using commercially-available kits.
2. Submit DNA samples to the UW-Madison Biotechnology Center for PCR amplification, Illumina
next-generation sequencing, and bioinformatics analyses to compare microbial populations
among sampling sites within a system, among systems at a single production site, and among
production sites in the North-Central Region (NCR).
3. Develop a fact sheet explaining the importance of microbial populations in an aquaponics system,
including the risk that certain organisms can cause (e.g., plant, fish, and human pathogens),
develop a recorded presentation for dissemination using the NCRAC website, and produce oral
presentations and a peer-reviewed manuscript.
|Matthew Rogge (Univ. of WI-Stevens Point||1, 2, 3||$18,596|
|Nicholas Phelps||1, 3||$4,500|
|Danial Graf||2, 3||$1,500|
Project Number: 1
Award Amount: $24,596
Principal Investigator: Matthew Rogge