Description:
We propose to use metagenomic DNA sequencing techniques to characterize populations of microbes in aquaponics systems in the Midwest.
Chaired by Matthew Rogge
Objectives:
1. Collect microbial samples from multiple sites within an aquaponics system and from various systems across a
geographic range and extract bacterial DNA using commercially-available kits.
2. Submit DNA samples to the UW-Madison Biotechnology Center for PCR amplification, Illumina
next-generation sequencing, and bioinformatics analyses to compare microbial populations
among sampling sites within a system, among systems at a single production site, and among
production sites in the North-Central Region (NCR).
3. Develop a fact sheet explaining the importance of microbial populations in an aquaponics system,
including the risk that certain organisms can cause (e.g., plant, fish, and human pathogens),
develop a recorded presentation for dissemination using the NCRAC website, and produce oral
presentations and a peer-reviewed manuscript.
Participant(s):
| Principal Investigators/Institution | Objectives | Total |
| Matthew Rogge (Univ. of WI-Stevens Point | 1, 2, 3 | $18,596 |
| Nicholas Phelps | 1, 3 | $4,500 |
| Danial Graf | 2, 3 | $1,500 |
| Total | $24,596 |
Project Number: 1
Award Amount: $24,596
Principal Investigator: Matthew Rogge