Development of an all-female yellow perch population: A strategic approach using thermal manipulation, sperm selection, and genomic data analysis

Project

Description:

Development of an all-female yellow perch population: A strategic approach using thermal manipulation, sperm selection, and genomic data analysis

Chaired by Osvaldo J. Sepulveda Villet

Objectives:

  1. To determine the influence of temperature on gonadal differentiation in yellow perch of Ohio origin raised at low 14oC (57oF) or high 24oC (75oF) water temperature from fertilization until completion of sex differentiation.
  2. Examine the sex ratio and growth rate of progenies sired by potentially sex reversed males (obtained from objective 1) reared in parallel groups (OSU) and separately in a “common garden” design by factorial crossing (UW-M). Additionally, outcross performance (fertilization, survival, growth rates at 30 and 90 days, feed efficiency) will be evaluated among crosses of Ohio strain and hybrids between UW-M genetically improved yellow perch x Ohio perch sperm.
  3. To determine if the use of a flow-cytometry-based cell sorting method will correctly identify and segregate “Y”- and “X”-sperm, using a fluorescent nuclear tag and differential fluorescence as separation criteria (UW-M).
  4. To characterize DNA from “X”-sperm and utilize a novel yellow perch genome to identify putative sex-linked markers that can be used to increase efficiency of cell-sorting or other molecular-based sperm selection methods (UW-M).
  5. To optimize high-throughput cryopreservation methods for yellow perch sperm and develop a pilot cryo-bank of sex-reversed (“XX”) male yellow perch sperm, which will be immediately available for use by fish farmers in the North-Central region to produce all-female progenies for grow-out (OSU).
    Institution/Company PI Objectives Year
    1
    Year
    2
    Total
    Univ. of WI -Milwaukee Osvaldo J. Sepulveda Villet 2, 3,4 $45,664 $49,079 $94,743
    Ohio State Univ. Konrad Dabrowski 1,2, 5 $33,859 $33,659 $67,518
    TOTAL     $79,523 $82,738 $162,261

    Project Outline

Duration: 
07/01/2017 to 06/30/2019
Award Amount: 
$162,261.00
Award Number: 
2014-28500-25753
Category: 
Project Type: